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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Hepatology. 2011 Nov 30;55(1):267–276. doi: 10.1002/hep.24681

Fig. 1. Activation by TCA of S1P2 expressed in HEK293 cells.

Fig. 1

A. Representative images of the GFP-tagged S1P1 and S1P2 stably expressed in HEK293 cells. B. Effect of TCA on ERK1/2 activation. HEK293 cells expressing GFP-tagged S1P1 or S1P2 or GFP only were treated with TCA (5 or 50 µM) or vehicle control for 20 minutes at 37°C. The protein levels of phosphor-ERK1/2 and total-ERK1/2 were determined by Western blot analysis. *p<0.05 compared to vehicle control, n=3.