Skip to main content
. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Hepatology. 2011 Nov 30;55(1):267–276. doi: 10.1002/hep.24681

Fig. 4. Effect of JTE-013 on conjugated bile acid-induced activation of ERK1/2 and AKT in primary rat hepatocytes.

Fig. 4

Cells were pre-incubated with JTE-013 (10 µM) for 30 minutes and then treated with individual conjugated bile acids (TCA, TDCA, TUDCA, GCA, GDCA, 50 µM) or S1P (100 nM) for 30 minutes at 37°C. The protein levels of phosphorylated ERK1/2 (p-ERK1/2) and AKT (p-AKT) were determined by Western blot analysis and normalized with total ERK1/2 (T-ERK1/2) and total AKT (T-AKT) as described in Materials and Methods. The representative Western blot images are shown. The relative density was determined by Image J software.