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. Author manuscript; available in PMC: 2012 Dec 23.
Published in final edited form as: Angew Chem Int Ed Engl. 2011 Nov 9;50(52):12534–12537. doi: 10.1002/anie.201105065

Figure 1. Fluorescence polarization-based assays for STs and FUTs (Option 1).

Figure 1

(a) Sialyltransferase (SiaT) assay based on transfer of FITCNeuAc from CMP-FITCNeuAc to N-linked (ST6Gal I, ST3Gal III) and O-linked (ST3Gal I) glycans of asialo-fetuin. (b) Fucosyltransferase (FUT) assay based on transfer of a fluorescent fucose analog from GDP-FLUORFuc to an N-glycan on fetuin. (c) Time course of ST3Gal III-mediated transfer of FITCNeuAc to asialo-fetuin produces increased fluorescence polarization over time, and is inhibited by the known competitive inhibitor, CDP. (d) Time-course of the FUT7 fluorescence polarization assay using GDP-FLUORFuc, fetuin, and FUT7, and inhibition by the known competitive inhibitor GDP.