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. 2004 Feb 1;113(3):441–450. doi: 10.1172/JCI20267

Figure 1.

Figure 1

Phosphorylation of Akt and GSK-3β in WT, Akt1–/–, and Akt2–/– mice. Washed platelets (2 × 107 platelets in 100 μl) were stimulated for 10 minutes with buffer alone (untxd), thrombin (thr; 1 U/ml), or thrombin in the presence of the PI3K inhibitor, LY294002 (thr + LY; 50 μM) and then immunoblotted with Ab’s directed against (a) Akt phosphorylated on serine 473 (Akt-PSer473), (b) total Akt, (c) GSK-3β phosphorylated on serine 9 (GSK-3β–PSer9), and (d) total GSK-3β. The results shown are representative of those obtained a minimum of three times.