Fig. 1.

A. Construct containing PMI marker gene under the control of Act1 promoter and SQS gene with maize UBI1-13 promoter. (LB: left border; RB: right border)B. PCR amplification of SQS in transgenic RNAi lines (lane 1 and 2) with WT in well 1. Lane 3 and 4 represent the PCR amplification of genomic DNA for internal control gene CBR (cytochrome b5 reductase) C. RT-PCR analysis of abundance of endogenous SQS transcript in WT and three independent RNAi lines. Equal amounts of RNA was extracted from WT and RNAi lines and were used to synthesize 1st strand cDNA. 3μl of this cDNA was amplified using gene-specific primers (see materials and methods for details) for SQS D. RT-PCR analysis of rice ubiquitin5 gene with 3μl template was used as the housekeeping control.