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. 2011 Sep 21;63(1):177–190. doi: 10.1093/jxb/err259

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© 2011 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 1. — Electrochemical detection of H₂O₂ and NO production from Arabidopsis epidermis in response to Ca²⁺_o. (A, B) Calibration of the H₂O₂- and NO-selective electrochemical sensors ISO-HPO (A) and ISO-NOP (B). The current output jumped rapidly after each addition of H₂O₂ of known concentration or SNAP in CuCl₂ solution that produced a known concentration of NO. The calibration curve is shown in the inset. (C, D) Trace obtained upon supplementation with 10 mM (upper trace) or 50 μM (lower trace) CaCl₂ in epidermal peel suspensions using ISO-HOP (C) and ISO-NOP (D). Each trace represents the average of three samples (±SE). The arrow shows the time point at which CaCl₂ was applied.