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. 2011 Sep 21;63(1):177–190. doi: 10.1093/jxb/err259

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© 2011 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — Ca²⁺_o-induced H₂O₂ accumulation in guard cell chloroplasts from Arabidopsis. (A, B) TEM images of chloroplasts in guard cells with CeCl₃ staining from a leaf section incubated in 50 μM (A) or 2 mM (B) CaCl₂. (C) Further detail of A. (D) Further details of B. The black spots in the TEM images represent H₂O₂ forming electron-dense cerium perhydroxide precipitates. Examples of individual precipitates are shown by arrows. s, stroma; t, thylakoids. Bar=200 nm. (E) Confocal images of changes in H₂DCF fluorescence intensity from guard cell chloroplasts of the wild type in response to 50 μM (control) and 2 mM CaCl₂. H₂DCF, chlorophyll, and overlay fluorescence images from guard cell are shown. Bar=10 μm. (This figure is available in colour at JXB online.)