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. 2011 Sep 21;63(1):177–190. doi: 10.1093/jxb/err259

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© 2011 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 4. — H₂O₂ and NO productions occur before stomatal closure induced by Ca²⁺_o in Arabidopsis. (A, B) Kinetics of the increase in H₂DCF (A) and DAF-FM (B) fluorescence intensities in guard cells of both wild type and CASas in response to 2 mM Ca²⁺_o (n=50, ±SE). The relative changes in H₂O₂ and NO production were expressed using the fluorescence at time zero as the standard (100%). (D, E) Examples of H₂DCF (D) and DAF-FM (E) fluorescence images of guard cells from A and B, respectively. Bar=50 μm. (C) Kinetics of 2 mM Ca²⁺_o-, 10 μM H₂O₂-, and 60 μM SNP-induced stomatal closure of both the wild type and CASas (n=100, ±SE). Stomatal apertures (width to length) were measured as the percentage using zero time as the standard (100%). (This figure is available in colour at JXB online.)