Fig. 3.

Dynamics of cytoplasm stained with soluble GFP in the presence of dominant negative myosin tail constructs fused to RFP. Nicotiana benthamiana leaves were infiltrated with a construct encoding GFP alone or GFP with an RFP fusion of coiled-coil tail domains from MYA2 or XIK (shown in magenta). First, one image was acquired including the two channels to ensure that both colours are present in the same cell (left and middle images) and then a time-lapse movie was acquired using only the GFP channel. Images were acquired every 2 s for 30 frames. Frames 0, 10, and 20 are coloured green, magenta, and blue, respectively and projected on each other (right images). White represents co-localization of the three colours in the same pixel. Scale bar=10 μm.