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Serial deletions and point mutations in the globular tail domain (GTD) of N. benthamiana myosin XIK. (A) Scheme of the deletion mutants (S1–S7). Black boxes show the predicted helixes. Numbers indicate the first and last amino acid in each deletion. The two point mutations (R1368A/P and R1443A) shown by arrows were introduced into fragments spanning the whole GTD. (B) Western blot analysis of all deletion mutants. Proteins were detected using anti-GFP antibodies.
