Table 2.
Enzyme activities of extracts from membrane-bound fractions of immature green (IMG) and red (RD) Micro-Tom fruits. One unit of enzyme activity is defined as the number of micromoles of NADPH oxidized [for D-galacturate reductase (D-GalAR) activity] or p-nitrophenol decolourized by L-galactono-1,4- lactone, L-galactonic acid, l-gulono-1,4-lactone, and D-glucono-1,5-lactone [for aldonolactonase (Alase) activity]. Values are the mean ±SE of three independent determinations.
Substrate | IMG | RD |
D-GaIAR activity | ||
D-Galacturonic acid | 22.02±0.86 a | 26.18±0.88 b |
Alase activity | ||
L-Galactono-1,4-lactone | 5.55±0.51 a | 12.38±1.38 b |
L-Galactonic acid | 10.76±1.96 a | 21.39±2.97 b |
L-Gulono-1,4-lactone | 16.19±0.89 a | 15.87±1.65 a |
D-Glucono-1,5-lactone | 45.13±3.07 a | 45.36±3.78 a |
Values with different letters are significantly different according to t-test (P <0.05).