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. 2011 Sep 21;63(1):275–292. doi: 10.1093/jxb/err278

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© 2011 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 5. — Leaf senescence induced by continuous darkness is enhanced in AAF-OX. (A–C) Dark-induced senescence was analysed by using detached leaves from 4-week-old plants. The fifth and sixth leaves were excised and incubated in darkness for 5 d followed by measuring the chlorophyll content (A), photochemical efficiency (B), and membrane ion leakage (C). *P <0.01, 5 d versus 0 d (n=6); †P <0.01, AAF-OX or aaf-KO versus the wild type (Col-0) by the percentage of chlorophyll reduction after dark treatment (n=6). Data represent means ±SE. (D) Electroscopic analysis of 3-week-old plants treated with continuous darkness for 5 d (ii and iv; wild type and AAF-OX, respectively) or without dark treatment (i and iii; wild type and AAF-OX). Bars=0.5 μm.