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Expression of AAF is induced by ACC. Expression levels of AAF (A) and ERF1 (B) were quantified by real-time PCR in 7-day-old light-grown seedlings of Col-0 and ein2-5 in the absence and presence of 100 μM ACC. Samples of total RNA were prepared from seedlings collected at the indicated times. Data were normalized to the expression of ACTIN2 (At3g46520). Fold change of Col-0 at 0 day was set as 1. *P <0.01 versus the wild type, Col-0 at 0 d. Data represent means ±SE (n=3).
