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. 2011 Sep 21;63(1):293–304. doi: 10.1093/jxb/err279

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© 2011 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 6. — Transcript levels of CBF1, CBF3, and different cold-inducible genes in acex55 and acex506 mutants. RNA-blot hybridizations were performed with total RNA isolated from 4-week-old Col (WT) and mutant plants grown under control conditions (C) or exposed to 4 °C for the indicated times. Specific probes for CBF1 and CBF3, as well as for CBF target genes COR15A, COR47, KIN1, and LTI78, and the non-CBF-target gene RCI2A were used for hybridizations. The transcript levels of CBF2 in the mutants are shown as internal controls. Similar amounts of RNA were present in each sample as confirmed by ethidium bromide staining of rRNA.