Fig. 2.

The GRXS13.2 gene variant is induced by methyl viologen (MeV) and high light (HL) stress treatments. Transcript levels for GRXS13.1 and GRXS13.2 (A, B) and GST-1 (C, D) were measured using qRT-PCR in 15-day-old Arabidopsis seedlings at different times (in hours) after treatments (black bars) with 50 μM MeV (A, C) or with HL (1000 μmol m⁻² s⁻¹) (B, D). Control treatments were performed in MS medium (white bars). Transcript levels of GRXS13.1 and GRXS13.2 variants were normalized by comparison with a reference gene, and the extent of gene induction was calculated with regard to the basal level (time 0) for the corresponding variant. Clathrin was used to normalize GRXS13.1 and GRXS13.2 expression, while UBQ10 was used to normalize GST-1 expression. Data corresponds to mean values ±SD from three biological replicas. Statistical analysis was performed using the paired Mann–Whitney test with the GraphPad Prism 5 program. Values showing statistically significant differences relative to the corresponding WT samples are indicated by asterisks (*P < 0.05 and ***P < 0.001).