Table 4.
Comparison of microarray and qRT-PCR results for selected genes
| Early/2 months | Microarray | qRT-PCR | |
|---|---|---|---|
| Gene Symbol | Fold-change | Fold-change | p-value |
| Cxcr3 | 1.534 | 1.604 | 7.13E-05 |
| Eomes | 1.460 | 1.610 | 1.29E-02 |
| FoxP1 | -1.326 | -1.512 | 3.30E-04 |
| Gzmm | 4.297 | 11.452 | 5.41E-06 |
| IL18r1 | 1.399 | 2.096 | 2.15E-03 |
| IL2rb | 1.566 | 1.415 | 6.03E-03 |
| Late/12 months | Microarray | qRT-PCR | |
| Gene Symbol | Fold-change | Fold-change | p-value |
| Bub1b | 1.618 | 1.423 | 3.82E-07 |
| CD8b1 | 2.541 | 1.889 | 2.76E-02 |
| Eomes | 2.327 | 2.073 | 1.28E-02 |
| FoxP1 | -2.593 | -2.045 | 9.80E-05 |
| Gzmm | 10.424 | 24.514 | 8.60E-03 |
| IL18r1 | 2.085 | 2.042 | 2.88E-02 |
| IL2rb | 1.821 | 1.516 | 3.61E-02 |
A subset of genes identified as differentially expressed by microarray analysis were validated by qRT-PCR at both the early and late time points. The fold-change found by microarray and qRT-PCR were similar for all genes assessed by qRT-PCR.