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. 2011 Aug 12;12(8):R74. doi: 10.1186/gb-2011-12-8-r74

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Copyright ©2011 Mehta et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Myosin staining pattern in quiescent HGPS human dermal fibroblasts after FTI treatment and following re-stimulation. AG11498 HDFs treated with FTI-277 were serum starved for 7 days to induce quiescence. The cells were then re-stimulated with fresh serum and samples were collected at 0, 24, 36 and 48 hours after serum restoration. Samples were also collected before serum withdrawal (proliferating cells). The samples were then fixed with methanol:acetone (1:1) and distribution of NM1β was assessed by performing an indirect immunofluorescence assay for NM1β. (a, c, e, g, i) The distribution of NM1β in cells before and after restimulation of quiescent fibroblasts. (b, d, f, h, j) DAPI staining. Scale bar = 10 μm.