Table 1.

Distribution of NM1β in HGPS cells

	Proliferating HGPS cells	0 hours (quiescent HGPS cells)	24 hours	36 hours	48 hours
Nucleolar + rim + nucleoplasmic	5.8 ± 3.0	0.8 ± 2.0	2.7 ± 6.24	3.1 ± 3.06	10.4 ± 3.05
Nucleolar only	0 ± 0	19.8 ± 6.65	19.7 ± 6.506	8.0 ± 6.08	8.2 ± 3.05
Aggregates	85.1 ± 6.11	77.3 ± 6.55	71.9 ± 5.85	83.3 ± 3.06	76.4 ± 8.96
Rim + nucleoplasmic	5.2 ± 2.51	0 ± 0	2.2 ± 3.06	2.9 ± 4.0	2.2 ± 3.61
Nucleoplasmic	2.9 ± 2.64	1.4 ± 3.51	1.8 ± 3.51	1.8 ± 4.0	1.8 ± 2.64
Dull	0.1 ± 1.53	0.6 ± 0.57	1.7 ± 3.78	0.7 ± 2.0	0.9 ± 2.51

Proliferating HGPS cells were in 15% serum, quiescent HGPS cells were in 0.5% serum for 7 days, and 24, 36 and 48 hours refer to time after the re-addition of serum. The distribution of NM1β is normally nucleolar + rim + nucleoplasmic in proliferating control cells, while abnormal distributions are nucleolar only, aggregates, rim + nucleoplasmic, nucleoplasmic only and dull. In control quiescent cells aggregates are the most common distribution observed [42]. Numbers are percentage values ± standard deviation. The most prominent distribution of NM1β in HGPS cells in high and low serum was in aggregates.