Figure 6.

Classes of dynamically regulated genes during planarian regeneration. (a, b) Venn diagrams depicting the number of statistically significantly regulated genes detected during the Illumina transcriptome sequencing (RNAseq) time course (adjusted P-value < 0.001, and log₂ fold change > 0.7 or < -0.7) at one or more time points. Colors indicate 0.5 to 1 h samples (blue; included only in (a)), 2 to 3 h samples (yellow), 4 to 8 h samples (purple), 10 to 18 h samples (green) and 24 to 72 h samples (red; included only in (b)). (c) Heatmap display of the consensus results from 100 k-means clustering runs with z-score transformed expression changes of significantly regulated genes (P-value and log₂ fold change cutoff as in (a)). Rows correspond to genes, columns correspond to Illumina RNAseq time points. The color scale ranges from negative (blue) through neutral (black) to positive z-scores (yellow). Genes discussed in the main text are indicated by arrowheads.