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. 2011 Dec 23;4:55. doi: 10.3389/fnmol.2011.00055

Figure 3.

Figure 3

Chinese hamster ovary-MAG cells inhibit CGN neurite outgrowth, which can be partially reversed by the ROCK inhibitor Y-27632. (A) Quantification of the mean neurite length per transfected neuron of CGNs cultured on CHO-MAG or CHO-R2 cells. The mean neurite length per transfected CGN was significantly longer when cultured on CHO-R2 cells compared to CHO-MAG cells. The CHO-MAG inhibition could be partially reversed with 20 μM Y-27632. The mean neurite length per transfected CGN was significantly longer when cultured on CHO-MAG cells in the presence of Y-27632 compared to CGNs cultured on CHO-MAG cells without Y-37632. Values represent mean and SD; analysis was performed using one way ANOVA with Dunnett’s post hoc tests comparing to CHO-MAG cells (***P < 0.001), n = 8. (B) Non-inhibitory CHO-R2 cell substrate. (C) CGN neurite outgrowth is inhibited by the CHO-MAG cell substrate. (D) The ROCK inhibitor Y-27632 reverses the MAG inhibition and promotes neurite outgrowth. Scale bar: 100 μm.