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. 2011 Dec 23;4:55. doi: 10.3389/fnmol.2011.00055

Figure 4.

Figure 4

CSPG inhibits postnatal CGN neurite outgrowth, which can be partially reversed by the ROCK inhibitor Y-27632. (A) Quantification of the mean neurite length per transfected neuron of CGNs cultured on Laminin and CSPG substrates. CGNs cultured on laminin had significantly longer neurites than CGNs cultured on a CSPG substrate. The CSPG inhibition could be partially reversed using 20 μM Y-27632. The mean neurite length per transfected CGN was significantly longer when cultured on the CSPG substrate in the presence of Y-27632 compared to CGNs cultured on the CSPG substrate without Y-37632. Values represent mean and SD; analysis was performed using one way ANOVA with Dunnett’s post hoc tests comparing to CSPG substrate (***P < 0.001, **P < 0.01), n = 8. (B) Non-inhibitory laminin substrate. (C) Neurite outgrowth is inhibited by the CSPG substrate. (D) The ROCK inhibitor Y-27632 can promote CGN neurite outgrowth and overcome the CSPG inhibition. Scale bar: 100 μm.