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. 2011 Dec 23;4:55. doi: 10.3389/fnmol.2011.00055

Figure 6.

Figure 6

Regeneration-associated genes are successfully over-expressed following electroporation, and over-expression of KLF-7 but not ATF-3 was able to enhance neurite outgrowth on an inhibitory MAG substrate. (A) Quantification of the relative level of ATF-3 mRNA in electroporated CGNs. Significantly higher levels (2700-fold) of ATF-3 were detected in CGNs transfected with the ATF-3 plasmid compared to CGNs transfected with the mCherry plasmid. Values represent mean and SD; analysis was performed using an independent samples Student’s t-test (**P < 0.01), n = 4. (B) Quantification of the mean neurite length per transfected neuron of CGNs cultured on CHO-MAG cells. The CHO-MAG inhibition could be partially reversed by over-expressing KLF-7 or addition of 20 μM Y-27632 but not by over-expressing ATF-3. Values represent mean and SD; analysis was performed using one way ANOVA with Dunnett’s post hoc tests comparing to CHO-MAG cells (***P < 0.001), n = 8.