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. 2011 Nov 18;12(12):1265–1272. doi: 10.1038/embor.2011.212

Figure 3.

Figure 3

Opm is required for Wg secretion in vivo. (A,B) Total and extracellular Wg staining of larval wing discs of the indicated genotypes (n>5 for all genotypes). (C,D) Total Wg protein staining of larval wing discs colabelled with KDEL-GFP. Quantification of the degree of colocalization for n=3 was done through the JACoP plugin in ImageJ, resulting in an average Pearson's coefficient of P=0.56 and P=0.68 for rel-RNAi and opm-RNAi, respectively, which supports an enhanced colocalization in the ER on opm-RNAi. To complement the analysis, Mander's correlation coefficients were calculated (M=0.47 for rel-RNAi versus M=0.59 for opm-RNAi). (E,F) Total Wg protein staining of third-instar larval wing discs co-stained with GM130 (n=5 for all genotypes). ER, endoplasmic reticulum; Evi, Evenness interrupted; GFP, green fluorescent protein; opm, opossum; RNAi, RNA interference; Wg, Wingless.