FIG. 5.

The two validation settings of the osteochondral culture model. (A) Validation setting 1: H&E-stained osteochondral biopsies with defects of chondral (1), subchondral (2), and osteochondral (3) depth; scale bars indicate 3 mm. (B) Validation setting 1: safranin–O–stained detail images of tissue formed by direct seeded chondrocytes in defects of chondral (1), subchondral (2) and osteochondral (3) depth; scale bars indicate 400 μm. (C) Validation setting 1: scoring of volume and integration of newly formed tissue on safranin-O–stained sections; mean with standard deviation (n=9); *indicates p=0.01. (D) Validation setting 2: direct seeding (1) versus membrane seeding (2) of bovine chondrocytes in osteochondral defects; scale bars indicate 400 μm. (E) Validation setting 2: scoring of volume (*indicates p=0.01) and integration (*indicates p=0.03) of newly formed tissue on safranin-O–stained sections; mean with standard deviation (n=9). NT, newly formed tissue; C, native cartilage; CC, calcified cartilage remains; M, chondrogide membrane. Color images available online at www.liebertonline.com/tec