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. Author manuscript; available in PMC: 2012 Oct 28.
Published in final edited form as: Immunity. 2011 Sep 29;35(4):526–535. doi: 10.1016/j.immuni.2011.09.005

Figure 2. Hybridomas expressing mouse DO-11.10 TCRα plus DO-11.10 TCRβ with mouse, frog, trout, and shark Vβ respond to cells expressing mouse MHCII IAd plus OVA peptide.

Figure 2

A. Schematic of chimeric TCRβ construction replacing mouse Vβ8.2 with frog, trout, and shark Vβ in the DO-11.10 TCRβ with full-length mouse TCRβ constant region for expression in hybridomas. (B and C) IL-2 production of 5KC-73.8.20 hybridomas expressing wild-type DOα (B) or S93A DOα (C) plus mouse DOβ, frog DOβ, trout DOβ, shark DOβ, or no TCRβ after stimulation with IAd-expressing A20.2J cells plus the indicated concentration of OVA323–339 peptide. Filled circles indicate wild-type TCRβ and open circles indicate Y48A TCRβ. Data in (B) and (C) are mean of 2 independent experiments, with a lower limit of detection of IL-2 at 50 pg ml−1.