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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Amino Acids. 2011 Aug 2;42(2-3):495–505. doi: 10.1007/s00726-011-1028-6

Figure 1. Transgene construct and PCR identification of transgenic mice.

Figure 1

A, The human SpdS cDNA was placed under the control of composite cytomegalovirus-immediate early gene enhancer/chicken β-actin (CAG) promoter elements to generate the transgene expression construct. An HA epitope was added to the amino terminus of the hSpdS to enable detection of transgene-derived SpdS protein. Arrows indicate the positions of primers used for PCR identification of transgenic mice in B. B, A representative analytic gel of tail DNA extracts from eight potential transgenic mice (lanes 2 to 9) and a DNA standard (lane 1). Genotyping by PCR yielded a 397 bp product from the genomic DNA from transgenic animals (lanes 2, 7 and 9). Primers that amplify a 520 bp product from the mouse antizyme gene were included in all reactions as a positive control.