Fig. 1.

Genetic interactions between eIF5A and eEF2. a Tenfold serial dilutions of wild type (eIF5A) cells harboring the vector alone, the mutant strains eIF5A^K56A or eIF5A^P83S harboring the vector alone, or high-copy eEF2 or high-copy Pkc1 plasmid were plated onto SC-ura to determine the growth at permissive (25°C) or non-permissive temperatures (35°C to eIF5A^K56A and 38°C to eIF5A^P83S). b The wild type strain harboring the vector alone or the eIF5A^K56A mutant strain harboring the vector alone or together with eEF2^H699K dominant negative mutant plasmid were assayed as described in (a). The plates were photographed after 3–4 days of growth