Skip to main content
. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Nat Neurosci. 2011 Nov 20;15(1):23–25. doi: 10.1038/nn.2962

Figure 2. A1R antagonists induce a long-lasting increase in synaptic strength in CA2 neurons in vitro.

Figure 2

Bath-application of caffeine (100 µM, a) or DPCPX (10 nM, c) for 5-min potentiates EPSCs in CA2, but not in CA1. Duration of drug perfusion is indicated by the gray bar in (a) and (c) and in subsequent panels. Inset traces recorded at the time-points marked by the numbers (calibration; 25 pA, 25 msec). The A1R-P in CA2, plotted as a function of stimulation intensity, persists in slices previously exposed to caffeine (b) or DPCPX (d) for 5-min and then returned to standard ACSF for either one, two or three hours prior to recording. A high concentration of caffeine potentiates responses in CA1 (300 µM, e and g). Similarly, The selectivity of the A1R antagonist CPT to potentiate responses in CA2 and not in CA1 is lost when a higher concentration is applied (100 nM versus 1 µM; f and h).