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. Author manuscript; available in PMC: 2012 Jun 22.
Published in final edited form as: Nature. 2011 Nov 6;480(7378):547–551. doi: 10.1038/nature10648

Figure 1. Induction and neurogenic conversion of hESC-derived midbrain FP precursors is dependent on CHIR99021 addition.

Figure 1

a) Immunocytochemistry at day 11 for FOXA2 (red), NESTIN (green, upper panels), LMX1A (green, middle panels) and OTX2 (green, lower panels). b,c) Quantification of the data presented in (a); mean ± SEM, n=3 (independent experiments): *** p < 0.001; ** p < 0.01; p < 0.05 (compared to LSB, Dunnett test). d) Diagram of culture conditions. e,f) Selected lists of differentially expressed transcripts at day 11 comparing LSB/S/F8/CHIR versus LSB (e) or versus LSB/S/F8 (f). g,h) Temporal gene expression analysis for markers of midbrain DA precursor (g), forebrain and ventral non-DA precursor identity (h). Scale bars: 50 μm.