Table 1.

Summary of exposure durations used in this study.

Assay	Start of Exposure	End of Exposure	Justification
Toxicity	8 hpf	120 hpf	Standard toxicity assay that encompasses entire organogenesis period
Larval behavior	8-10 hpf (note: larvae exposed from 8 to 58 hpf exhibit behavioral effects identical to those exposed from 10 to 58 hpf)	58 hpf	Narrowest window of exposure that leads to behavioral effects (narrow exposure window provides target organ clues); encompasses peak CNS development
Uptake/tissue dose	8 hpf	58 hpf	Intended to closely match the larval behavior test exposure
Adult behavior	8 hpf	120 hpf	Encompasses entire organogenesis period; intended to maximize chance of detecting behavioral effects in adults