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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Nat Neurosci. 2011 Dec 11;15(1):113–122. doi: 10.1038/nn.2993

Figure 3.

Figure 3

Requirements for the induction of ITDP. (a) Experimental design. (b) ITDP in cortical input was blocked by BAPTA (10 mM) in the recording pipette solution (n = 8, paired t test, P = 0.67 versus baseline). Insets show the average of fifteen cortico-LAn EPSCs before (1) and after (2) the TSt-CSt stimulation. Scale bars here and for other traces in the figure: 20 pA and 10 ms. (c) ITDP in cortical input in the presence of D-AP5 (50 μM, n = 8, P < 0.01 versus baseline). (d) ITDP in cortical input in the presence of nitrendipine (20 μM, n = 8, P < 0.05 versus baseline). (e) ITDP in cortical input was blocked in the presence of UBP 296 (5 μM, n = 6, P = 0.23 versus baseline). (f) Joint application of CPCCOEt (40 μM) and MPEP (20 μM) also blocked ITDP (n = 7, P = 0.61 versus baseline). (g) Xestospongin-C (10 μM) in pipette solution blocked the induction of ITDP (n = 7, P = 0.39 versus baseline). (h) Inclusion of ryanodine (100 μM) in pipette solution had no effect on ITDP (n = 7, P < 0.05 versus baseline). (i) Summary of ITDP experiments. Numbers within bars indicate the number of experiments for each condition. *P < 0.05, **P < 0.01, and ***P < 0.001, mean baseline EPSC amplitude versus EPSCs recorded 35–40 minutes after the TSt-CSt pairing, paired t test. Error bars indicate s.e.m.