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. 2011 Nov 8;82(5):1071–1085. doi: 10.1111/j.1365-2958.2011.07872.x

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© 2011 Blackwell Publishing Ltd

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Fig. 2 — TraI fragments including the relaxase domain and TSA are necessary and sufficient for nucleoprotein uptake. R17 RNA yield (A) and phage-induced cell lysis (B) are shown for plasmid R1-16 (•) or the R1-16ΔtraI (○) derivative when complemented with a wild-type R1 traI allele (▾) or fragments thereof. High RNA yields and cell lysis were observed with TraI N_1-992 (relaxase + TSA) (). No complementation was observed with a combination of TraI fragments N_1-309 (relaxase) and N_310-1756 (helicase) (▵) or fragment N_310-1756 alone (□). Values represent the mean of at least three experiments. Standard deviations are shown. Complementation of R1-16ΔtraI with TraI N_1-992 supports normal progression of phage replication as shown by electron microscopy of the infected cells (arrows) (C). In D an enlarged region of the distinctive honeycomb pattern of R17 is highlighted.

Inline graphic — TraI fragments including the relaxase domain and TSA are necessary and sufficient for nucleoprotein uptake. R17 RNA yield (A) and phage-induced cell lysis (B) are shown for plasmid R1-16 (•) or the R1-16ΔtraI (○) derivative when complemented with a wild-type R1 traI allele (▾) or fragments thereof. High RNA yields and cell lysis were observed with TraI N_1-992 (relaxase + TSA) (). No complementation was observed with a combination of TraI fragments N_1-309 (relaxase) and N_310-1756 (helicase) (▵) or fragment N_310-1756 alone (□). Values represent the mean of at least three experiments. Standard deviations are shown. Complementation of R1-16ΔtraI with TraI N_1-992 supports normal progression of phage replication as shown by electron microscopy of the infected cells (arrows) (C). In D an enlarged region of the distinctive honeycomb pattern of R17 is highlighted.