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TraI N1-992-catalysed nic-cleavage is stimulated by TraD. Conversion of supercoiled oriT DNA to the open circular (OC) form by purified TraI fragment N1-992 is expressed as per cent of total DNA substrate. Nic-cleavage was measured with 100 nM TraI N1-992 alone or with increasing concentrations of TraD. Values represent the mean of at least three experiments. Standard deviations are shown.
