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. Author manuscript; available in PMC: 2011 Dec 25.
Published in final edited form as: Methods Enzymol. 2010;482:167–183. doi: 10.1016/S0076-6879(10)82006-3

Fig. 2.

Fig. 2

A graphical interface is shown for writing the IHRSR Spider script. All of the needed parameters are entered through this interface, including the initial estimate of the helical symmetry (the rotation and axial translation that operates on one subunit to generate another one). For objects with a rotational point group symmetry Cn in addition to a helical symmetry, the value of n can be entered.