Figure 2.
δF508-CFTR corrector activity and cellular uptake of 5. (A) Short-circuit measurements showing apical membrane chloride current after incubation for 24 h at 37 °C with 10 µM of indicated fluorescent bithiazoles. Negative control was incubation with DMSO vehicle alone and positive control was incubation with corr-4a (5 µM). Forskolin (20 µM), genistein (50 µM) and CFTRinh-172 (10 µM) were added as indicated. (B) Confocal micrographs of live FRT cells after incubation with 5 for 1 or 15 min; for comparison, ER staining shown of FRT cells expressing an ER-targeted GFP. Scale bar, 5 µm.