Figure 1. Distinct functions of CD45 and CD148 in bacterial clearance.

(A) Mortality curves of mice of the indicated genotypes are shown, where n = 10 for each genotype. DKO refers to Ptprc^−/− Ptprj^TM−/TM− double mutant mice. DKO** were DKO mice treated with food tablets containing amoxicillin (3 mg), Flagyl (0.69 mg) and bismouth (1.185 mg) (1 tablet/cage/week). The difference between DKO and DKO** group is statistically significant (p<0.0001). (B) Animals of indicated genotypes were infected with S. aureus in the air pouch. After 24hr infection, live bacteria in the air pouch were collected by lavage. Recovered Colony Forming Units (CFU) of S. aureus from each mouse were counted and shown. Each symbol represents an individual mouse. Small horizontal lines indicate the means. Bacterial CFU was normalized between experiments based on their bacterial input. CFU shown is following an initial 10² dilution. (C) Neutrophil accumulation in the air pouch after 24 hr of S. aureus infection. Neutrophil counts were determined as (cell count from infected air pouch) × (percentage of neutrophils, identified as CD11b⁺ and Gr1⁺ by flow cytometry). (D) Neutrophil recruitment 6 hr after injection of heat inactivated S. aureus. (C,D) Data are presented as mean ± SEM; p values were calculated by the student t test. For (B) and (C), n=16 wild type mice, n=5 Ptprc^−/− mice, n=12 Ptprj^TM−/TM− mice, n=5 DKO mice were used for experiments. For (D), n=9 wild type mice, n=8 Ptprc^−/− mice, n=9 Ptprj^TM−/TM− mice, n=6 DKO mice were used for experiments.