Skip to main content
. Author manuscript; available in PMC: 2012 May 23.
Published in final edited form as: Immunity. 2011 Nov 10;35(5):757–769. doi: 10.1016/j.immuni.2011.09.011

Figure 7.

Figure 7

(A) BM cells (40×106/sample) were harvested and then lysed. Hck or Lyn was immunoprecipitated and subject to immunoblotting with antiphosphotyrosine (pTyr) Abs or anti-Hck and Lyn for loading controls. Specificities of Hck and Lyn Abs were confirmed with whole cell lysates from Hck−/− or Lyn−/− mice. Quantification of inhibitory tyrosine phosphorylation amounts were normalized to loading controls and the fold changes relative to wild type were graphed. For Hck n=3 and for Lyn n=4. (B) Neutrophils (15×106/sample) were treated with fMLP (3μm) for indicated times and then lysed. SHP-1 was immunoprecipitated and subjected to immunoblotting with antiphosphotyrosine (pTyr) Ab 4G10 or SHP-1 as a loading control. Data are representative of three independent experiments. See also Fig. S4.