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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Carbohydr Polym. 2012 Feb 1;67(2):1737–1743. doi: 10.1016/j.carbpol.2011.09.087

Figure 3. Removal of the unsaturated uronic acid residue of heparin by ozonolysis.

Figure 3

A. The proposed mechanism of ozonolysis of heparosan and heparin. In heparin R = [ → 4)-α-l-IdoA ± 2S(or β-d-GlcA)(1 → 4)-α-d-GlcNS±6Sand/or±3S(or GlcNAc±6S)(1 → ]n 4)-α-l-IdoA±2S(or β-d-GlcA)(1 → 4)-αβ-d-GlcNS±6Sand/or±3S(or GlcAc±6S). B. Preparation of LMWH by heparinase treatment followed by ozonolysis. 10 μg of LMWH prepared by heparinase, LMWH obtained by heparinase treatment followed by ozonolysis and pLMWH were separated by PAGE on a 22% acrylamide gel and stained with 0.5% (w/v) Alcian blue in 2% (v/v) acetic acid aqueous solution.