Fig. 1.
(A) Amino acid sequence alignment for region encompassing zebrafish (zERG) L499 with human (hERG), horse (eERG), dog (dERG), rat (rERG) and mouse (mERG). Domains S4, S5 and the S4–S5 linker are indicated below the sequences. The L at position 532 of hERG is highly conserved across species and is highlighted in gray. (B) Example current traces for WT (upper panel of Bi) and mutant L532P IhERG (upper panel of Bii) with corresponding voltage protocol used shown in the lower panels. Note that the current scales in Bi and Bii differ. Inset to Bii shows higher gain representation of L532P tail current.