Figure 1.

DNA methylation profiling in human embryonic stem cell (hESC) lines and normal primary tissues. (A) Matrix showing the number of demethylated CpG sites in each tissue type and demethylated CpG sites shared between different tissue types. (B) Illumina array methylation clustering heatmap of hESC-hypermethylated genes specifically demethylated in each of the eight normal tissue samples. Methylation levels vary from fully methylated (red) to fully unmethylated (green). (C) Box plots of microarray-based gene expression data (log scale). In each primary tissue, tissue-specific demethylated genes exhibited higher expression levels compared to other tissues. P-values are shown. n = number of genes analyzed.