Figure 3.
Expression of the NF-κB subunit p65 enhances DSB repair. (A and E) Homologous DSB repair was analysed in K562(Δ/3′) cells 48 h (A) and 72 h (E) after cells had been electroporated with pCMV-I-SceI, pcDNA3.0-p65/pcDNA3.0 and pcDNA3.0-IκBα-SR/pcDNA3.0 as indicated. Mean values from controls were defined as 100% (absolute mean values: 0.4 × 10−3 after 48 h, 0.9 × 10−3 after 72 h); Mean values and SEMs from n = 6; *P < 0.05. (B and F) In parallel experiments cells were treated with 25 μM z(VAD)-fmk. (C, D, G and H) Apoptosis (C and G) and cell cycle distribution (D and H) were analysed under the same conditions as in (A) and (E). Mean values and SEMs from n = 4. (I) Western blot analysis verifying p65 expression.