Figure 5.
Role of Bcl2-family proteins in NF-κB-mediated DSB repair regulation. (A, D and G) K562(Δ/3′) cells were co-transfected with pCMV-I-SceI, pcDNA3.0-p65 or empty vector plus a mixture of two different shRNA expression plasmids directed against Bcl2, Bcl-xL or BAX or empty vector. DSB repair frequencies were measured 72 h post-transfection; mean values of controls were taken as 100% each (absolute mean value 1 × 10−3). Columns, mean values of n = 6–12; bars, SEM; *P < 0.05; ***P < 0.001. (B, E and H) Cells were treated with 25 μM z(VAD)-fmk and homologous DSB repair was assayed as in (A, D and G). (C, F and I) Immunoblot analysis showing Bcl2, Bcl-xL and BAX knockdown.