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. 2011 Sep 13;40(1):428–437. doi: 10.1093/nar/gkr713

Figure 1.

Figure 1.

Inhibitory activity of Tra2 in pre-spliceosomal complex assembly. (A) Spliceosome assembly assays with a fushi tarazu (ftz) RNA splicing substrate were carried out in Drosophila Schneider 2 (S2) nuclear extract for the times indicated in the presence and absence of ATP. The positions of ATP-independent complexes (H), as well as ATP dependent A and C complexes are indicated. (B) Two 2′-O-methyl RNA oligos complementary to the U2 small nuclear RNA (snRNA) are tested for their effects on complex formation in the presence of ATP. The oligos were targeted at the loop region, essential for base pairing with the branchsite, and to the non-essential 20 nt at the 5′-end of the U2 snRNA. (C) Spliceosomal assembly assays on the ftz and ftz-ISS substrates were carried out with various amounts of recombinant Tra2 proteins in the presence of ATP. The dark bar in the schematic of ftz-ISS indicates the position of the 78-nt ISS element insertion in the ftz intron as previously described (27).