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. 2011 Sep 13;40(1):428–437. doi: 10.1093/nar/gkr713

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Dependence of tethered Tra2 function on binding position. Splicing of RNAs with MCP-binding sequences at different positions are compared. S2 nuclear extracts were supplemented with 100 or 400 nM of Tra2, MCP or MCP-Tra2 as indicated. The mobility of precursors and products are indicated to the side of each gel as in other figures. The percentage of total signal from splice products and intermediates is indicated below each lane. The positions of unspliced RNA (U), spliced product (S) and spliced intron (I) are indicated. (A) Two MS2-binding site inserted either 50-nt (ftzMS2-50) or 10-nt (ftzMS2-10) upstream of the branch site are tested for the effect of MCP-Tra2. (B) Two MS2 sites were inserted 40-nt downstream of the dsx female-specific 3′-splice site in the absence of the dsx splicing enhancer.