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. 2011 Sep 13;40(1):428–437. doi: 10.1093/nar/gkr713

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Exon and intron tethering of Drosophila SR proteins Rbp1 and ASF/SF2. In vitro splicing reactions with substrates dsxMS2 and ftzMS2-10 were carried out in S2 nuclear extracts supplemented with 400 nM MCP and 100 or 400 nM MCP-Tra2, MCP–Rbp1 and MCP–ASF/SF2 proteins. The positions of unspliced RNA (U), spliced product (S) and spliced intron (I) are indicated. The percentage of total signal from splice products and intermediates is indicated below each lane.