Figure 4.

Mapping of the translation initiator codons of VpALA1 and VpALA2. Wild-type and mutant ALA1 or ALA2 constructs were transformed into TRY11, and the ability of the constructs to rescue growth defects of the knockout strain was tested. (A) Schematic summary of the ALA1 and ALA2 constructs and their complementation activities. (B) Complementation assays for cytoplasmic AlaRS activity. (C) Complementation assays for mitochondrial AlaRS activity. (D) Analysis of cellular localization of VpAlaRS1 and VpAlaRS2 by fluorescence microscopy. A mitochondrial tracker and DAPI were used to label mitochondria and nuclei, respectively.