Figure 1.

Schematic of U1i and inhibition of Notch1 in vivo. (a) Schematic of U1-mediated inhibition. U1in are exogenous 5′-end modified U1 snRNPs designed to base pair to the 3′-terminal exon of a target gene. In this study, the U1in binds Notch1 or HBV mRNA and blocks expression by hindering polyadenylation (pA). (b) C57BL/6 mice were injected with pNFκβ-Luc plasmid (NFkBLuc) combined or not with plasmids expressing shαNotch1 (sh), U1inαNotch1 (U1in) or the combination of both. Luciferase activity was measured in living mice with a CCD camera at 4 days post-injection. Luciferase activity was quantified and plotted. The SI and the statistical significance are indicated.