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. 2011 Nov 15;40(1):e8. doi: 10.1093/nar/gkr956

Figure 5.

Figure 5.

Effect of U1ins and shRNAs targeting HBV in mice. C57BL/6 mice were injected with pCH-Fluc plasmid (HBVLuc) combined or not with plasmids expressing UB, UD, UE, UF and/or sh1 (a, c and e) or sh2 (b–e). Luciferase activity was quantified in living mice with a CCD camera at the indicated times post-injection (a–c) or in liver extracts obtained 8 days post-injection (e). PreS2/S Luc mRNA was quantified by RT–PCR from the same extracts (d). The relative number of copies of PreS2/S Luc mRNA obtained from 67 ng of pA + mRNA compared to a standard plasmid is shown. FI was calculated as described (c). The SI has been calculated for the combination of U1in and shRNA (c and d). RLU indicates relative light units (e). Data are mean ± SD from at least two independent experiments. Significant differences are indicated with asterisks. Statistical analysis shown compares animals treated with the combination of U1in and shRNA with animals treated with the best inhibitor alone (a, b and d) or with either of the inhibitors on its own (e). Statistical analysis of UB, UD and UE resulted in non-significant differences.