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(A) Melting temperature curve of ceuE qPCR products: (+) control: positive control; arrows represent ceuE melting curves of C. coli strains (4 different strains) and negative signals of non-C. coli strains (14 different strains), un-spiked samples, and water. (B) Agarose gel electrophoresis of qPCR products: Lane 1–4 (four different C. coli strains) with 196-bp band, lane M, 100-bp DNA marker; lane 5–18, 14 non-C. coli strains; lane 19, water; lane 20, un-spiked manure sample.
