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. 2011 Sep 26;14(1):6–18. doi: 10.1093/neuonc/nor141

Fig. 3.

Fig. 3.

Synthetic Pep-1 binds to IL-13Rα2. (A) Fluorescent peptide binding experiment on cells over-expressing IL-13Rα2 (SnB19-pcDNA) and cells with receptor knock down using anti-sense-IL-13Rα2 (SnB19-asIL-13Rα2). Pep-1-L and Pep-1-DSC as well as control linear and disulphide-constrained scrambled peptides (1 µg/mL and 5 µg/mL; 0.5 and 2.5 μM, respectively) were incubated with fixed cells for 1 h and detected using streptavidin-Alexa fluor 488. (B) Synthetic Pep-1 is internalized into GBM cells. Biotinylated peptides (5 µg/mL; 2.5 μM) were incubated with live U-251 MG GBM cells for 15 min and 4 h. The internalized peptides were detected using streptavidin-Alexa fluor 488. The cell nucleus was labeled with Topro-3 DNA dye. The pictures were taken using the LSM 510 confocal microscope. (C) Fluorescent peptide binding experiment on GBM tumor tissue and normal brain tissue. Biotinylated Pep-1-L and control scrambled peptide (10 µg/mL; 5.1 μM) were incubated with the cells and detected using streptavidin-Alexa fluor 488. The pictures were taken using the Olympus IX70 fluorescence microscope. The pictures are representative of three independent experiments.